Fig 1.

Purification of HCV E1E2 and selection of aptamers against E1E2 protein. (A) His-tagged E1E2 was expressed by IPTG induction in E. coli BL21(DE3) and confirmed using mouse anti-His monoclonal antibody via Western blot analysis. (B) Fluorescein isothiocyanate-labeled DNA pools from the control library, round 1, or round 8 were incubated with E1E2 or control LacZ protein in binding buffer. The density of the fluorescence was measured and normalized to that of the library. Error bars represent means ± standard deviations. **, P < 0.01 versus the library.