Fig 6.

The Q412R substitution in E2 is the major selective resistance substitution identified. Selection of a resistance-conferring substitution was performed. Huh7.5 cells were infected with medium from Huh7.5 cells transfected with RNA from the wild type (WT) or the selected Q412R viral clone. (A) The effect of aptamer E1E2-6 on RNA replication of the wild-type and the selected Q412R virus was examined as described in the legend of Fig. 3B. (B) The effect of aptamer E1E2-6 on the production of infectious particles of the wild-type or the selected Q412R virus was tested as described in the legend of Fig. 3E. The data are from three independent experiments. Error bars represent means ± standard deviations. *, P < 0.05; **, P < 0.01, versus control cells.