Fig 2.

Bactericidal activity of eCATH1 against R. equi residing inside murine peritoneal macrophages. Following mouse infection with a virulent strain of R. equi, peritoneal macrophages were collected. The infected macrophages were further treated with eCATH1 (20 μg/ml), rifampin at various concentrations, or a combination of both drugs (20 μg/ml eCATH1 plus 5 μg/ml rifampin). Survival of R. equi inside macrophages was assessed 24 h postinfection by CFU enumeration. Experiments were done in triplicate, and the results are expressed as means and standard deviations (SD); statistical significance in comparison to the control is shown as follows: ***P < 0.001; **** P < 0.00001.