Fig 2.

Effect of BZL on apical transporter expression in hepatic and extrahepatic tissues. P-gp (A) and Mrp2 (B) were detected by Western blotting in liver, small intestine, and kidney from rats treated with BZL (100 mg/kg b.w./day, 3 consecutive days) or vehicle (control [C]). Equal amounts of total protein (30 μg) were loaded in the gels. The ODs of P-gp and Mrp2 were related to the OD for β-actin. Uniformity of loading and transfer from gel to PVDF membrane were controlled with Ponceau S. Data on densitometric analysis are presented as percentages relative to the value for the control, considered 100%, and were expressed as means ± SDs (n = 4). Typical Western blot detections from each group are shown at the bottom. *, significantly different from control; (P < 0.05).