Fig 5.

Fosmidomycin-octaarginine salt complex inhibits extracellular T. gondii tachyzoites but does not affect intracellular replication. (A) Fluorescent micrographs of T. gondii-infected human foreskin fibroblasts (HFFs) showing incorporated FAM-octaarginine (green), anti-T. gondii antibody (red), and DAPI-stained DNA (blue). Plaque assay (B) and corresponding quantification (C) after treatment of T. gondii-infected HFFs with either 100 μM fosmidomycin, salt 2, or conjugate 3 are shown. Also shown are plaque assay (D) and corresponding quantification (E) after pretreatment of T. gondii tachyzoites with either fosmidomycin or fosmidomycin-octaarginine salt complex 2 prior to infecting new HFFs.