Fig 3.

Compound 1 is a potent inhibitor of enterovirus RNA replication. (A) BGM cells were infected with CVB3-Rluc at an MOI of 1. Following infection, the virus was removed and compound 1 or compound 2 was added to the cells. The values obtained with the replication inhibitor GuaHCl, used at 2 mM, are shown as a dashed line. (B) BGM cells were transfected with RNA transcripts of EV71 or CVB3 replicons. Immediately after transfection, the medium was replaced by fresh (compound-containing) medium. For the experiments in both panels A and B, at 8 h postinfection or posttransfection, cells were lysed to quantify the intracellular amount of luciferase as a measure of viral RNA replication. Data points or bars represent means of three samples ± the SD.