Fig 4.

Resistance of CVB3 mutants to compound 1. (A and B) BGM cells were infected with CVB3 wild type, CVB3 3A-V45A, or CVB3 3A-H57Y (A) or transfected with the corresponding RNA transcripts of full-length infectious CVB3 clones (B). Immediately after infection or transfection, various concentrations of compound 1 were added to the cells. After 8 h, cells were lysed by freeze-thawing to release intracellular virus particles, and the total virus titer was determined by endpoint titration. (B) Bars represent means of three samples ± the SD. Significant differences compared to wild-type virus are indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.