Fig 5.

Compounds 1 and 2 specifically inhibit PI4KIIIβ in vitro. Recombinant PI4KIIIβ or PI4KIIIα was incubated in the presence of compound 1 (upper panel) or compound 2 (lower panel) with their substrate, phosphatidylinositol, in the form of Triton micelles and radioactively labeled ATP. After termination of the enzyme reaction with phosphoric acid, the amount of radioactive ATP that was incorporated into the micelles was quantified as a measure of PI4K activity. Data were converted to the percent inhibition relative to controls. Data points represent the means of three samples ± the SD.