Fig 9.

Antiviral activity of compound 2 in a CVB4-induced pancreatitis mouse model. (A) Pancreas histopathology (H&E stain) in CVB4-induced pancreatitis. Mice were infected with CVB4, treated with compound 2 (right panel) or left untreated (left panel), and sacrificed 3 days p.i. (B) Histopathological severity scoring for CVB4-induced pancreatitis. H&E-stained tissue sections were scored blindly for inflammation, necrosis, and edema by using a standardized scoring system. A score of 0 indicates complete absence of pathology, whereas a score of 3 refers to diffuse lesions throughout the tissue section. (C) Effect of compound 2 on serum markers for pancreatitis. At day 3 p.i., serum was collected and lipase/amylase were quantified in enzymatic colorimetric assays. (D) Effect of compound 2 on infectious virus production, which was quantified by titration of tissue homogenates from cell cultures.