Table 1.

Broad-spectrum antienterovirus activity of compound 1

Virus group and species or strain	Cells	EC50 (μM)a	SIb
Human enterovirus A
EV71	BGM	0.011 ± 0.003	5,945
Human enterovirus B
CVA9	BGM	0.021 ± 0.004	3,114
CVB3	BGM	0.071 ± 0.018	921
ECHO9	BGM	0.054 ± 0.006	1,211
ECHO11	BGM	0.026 ± 0.007	2,515
Human enterovirus C
PV1	HeLa Rh	0.019 ± 0.008	2,505
PV2	HeLa Rh	0.013 ± 0.004	3,661
PV3	HeLa Rh	0.005 ± 0.002	9,520
Human enterovirus D
EV68	HeLa R19	0.031 ± 0.001	371
EV70	HeLa R19	0.025 ± 0.020	460
Human rhinovirus A
HRV2	HeLa Rh	0.009 ± 0.005	5,288
HRV9	HeLa Rh	0.006 ± 0.005	7,933
HRV15	HeLa Rh	0.021 ± 0.010	2,267
HRV29	HeLa Rh	0.005 ± 0.005	9,520
HRV39	HeLa Rh	0.004 ± 0.005	11,900
HRV41	HeLa Rh	0.017 ± 0.006	2,800
HRV59	HeLa Rh	0.006 ± 0.005	7,933
HRV63	HeLa Rh	0.006 ± 0.003	7,933
HRV85	HeLa Rh	0.024 ± 0.025	1,983
HRV89	HeLa Rh	0.024 ± 0.019	1,983
Human rhinovirus B
HRV14	HeLa Rh	0.031 ± 0.024	1,535
HRV42	HeLa Rh	0.027 ± 0.032	1,762
HRV70	HeLa Rh	0.024 ± 0.027	1,983
HRV72	HeLa Rh	0.028 ± 0.026	1,700
HRV86	HeLa Rh	0.024 ± 0.031	1,983

^aData represent mean values ± SD of at least three independent assays.

^bSI, selectivity index. The CC₅₀ was 65 μM for BGM cells, 47 μM for HeLa Rh cells, and 11 μM for HeLa R19 cells.