Fig 2.

Extracted ion chromatograms for RPV glucuronidated metabolites, M5, M6, and M7. Peaks not indicated as metabolites in these chromatograms were also present in the minus-NADPH and UDPGA controls and resulted in intensities below a signal-to-noise ratio of five. Human liver microsomes (2 mg/ml) were incubated with 20 μM RPV for 60 min at 37°C in the presence of an NADPH-regenerating system and UDPGA. Metabolites were detected by UHPLC-MS/MS in SRM mode using the following transitions: 543→367 m/z (M5), 559→383 m/z (M6), and 575→399 m/z (M7). Chromatograms are representative of three individual experiments. It should be noted that the y axes differ for each metabolite due to the observed intensity of the ions.