Table 1.

Chronological appearance of the mutations functionally characterized in S. pneumoniae R6M2B and 60827 and their transformants

Strain	CIP MIC (μg/ml)	Mutation
		parC	gyrA	patAa,b	patBc	spr0335	spr1544	spr1902
R6	0.5	WT	WT	WT	WT	WT	WT	WT
R6M2B	128	C245T	G253A	WT*	WT	WT	WT	WT
T1-R6M2B	16	C245T	WT	WT	WT	WT	A-28G	C248T
T2-R6M2B	64	C245T	G253A	WT	WT	WT	A-28G	C248T
T3-R6M2B	64	C245T	G253A	WT	WT	WT	A-28G	C248T
T4-R6M2B	64	C245T	G253A	WT	WT	WT	A-28G	C248T
T5-R6M2B	128	C245T	G253A	WT*	WT	C1246T	A-28G	C248T
T1-60827	2	WT	WT	RSS10	RSS10	WT	WT	C248T

^aPatA mutations identified in RSS10: A-33C, G335T, T537C, A540G, and C660T. A number preceded by a hyphen indicates the position of a mutation upstream of the ATG.

^bAn asterisk indicates an increased expression of patA as measured by quantitative real-time PCR.

^cPatB mutations identified in RSS10: T142G, T357C, T618C, T1260C, G1507A, and A1620G.