Table 3.
Functional analysis of mutations found by whole-genome sequencing in R6M2B, T5-R6M2B, and T1-60827
| Straina | Mutation |
CIP MIC (μg/ml)b | |||||||
|---|---|---|---|---|---|---|---|---|---|
| parC | gyrA | spr0129 | spr0335 | spr1544 | spr1902 | patA | patB | ||
| R6 | WT | WT | WT | WT | WT | WT | WT | WT | 0.5 |
| R6smR | WT | WT | WT | WT | WT | WT | WT | WT | 0.5 |
| R6spr1902-T5R6M2B, smR | WT | WT | WT | WT | WT | T5-R6M2B | WT | WT | 0.5 |
| R6parC-R6M2B | R6M2B | WT | WT | WT | WT | WT | WT | WT | 2 |
| R6parC,spr1902-T5R6M2B, smR | R6M2B | WT | WT | WT | WT | T5-R6M2B | WT | WT | 2 |
| R6parC,gyrA-R6M2B | R6M2B | R6M2B | WT | WT | WT | WT | WT | WT | 32 |
| R6parC,gyrA,spr1544-T5R6M2B,smR | R6M2B | R6M2B | WT | WT | T5-R6M2B | WT | WT | WT | 32 |
| R6parC,gyrA,spr1902-T5R6M2B,smR | R6M2B | R6M2B | WT | WT | WT | T5-R6M2B | WT | WT | 64 |
| R6patA,T1-60827,smR | WT | WT | WT | WT | WT | WT | T1-60827 | WT | 1 |
| R6patB,T1-60827,smR | WT | WT | WT | WT | WT | WT | WT | T1-60827 | 1 |
| T1-60827 | WT | WT | WT | WT | WT | WT | T1-60827 | T1-60827 | 2 |
| T5-R6M2B | R6M2B | R6M2B | WT | T5-R6M2B | T5-R6M2B | T5-R6M2B | WT | WT | 128 |
| T5-R6M2Bspr0335R6,smR | R6M2B | R6M2B | WT | WT | R6M2B | R6M2B | WT | WT | 64 |
| R6M2B | R6M2B | R6M2B | R6M2B | WT | WT | WT | WT | WT | 128 |
| R6M2Bspr0129R6,smR | R6M2B | R6M2B | WT | WT | WT | WT | WT | WT | 128 |
a
“smR” indicates that an rpsL+ allele conferring resistance to streptomycin was cotransformed along with the PCR fragment of interest for selection purposes. These strains are resistant to streptomycin.
b
MICs in bold are significantly different from the parent strain without the mutation.