Table 1.

Carbapenemase multiplex primers and probes

Gene	Primer/probe	Sequence (5′→3′)a	Reference	Nucleotide positions	Size (bp)
blaGIM-1	GIM1-F	CGACACACCTTGGTCTGAAGAA	AJ620678	1232–1253	81
	GIM1-R	GATGCTAGCCATAACCTGGTATCC		1313–1290
	GIM1-P	HEX-ACACGAAGTTGTTATTATCCTGGGCGACTGAC-BHQ-1		1255–1286
blaVIM-1	VIM1-F	TGCGCTTCGGTCCAGTAGA	FJ172675	698–716	76
	VIM1-R	TGACGGGACGTATACAACCAGAT		774–752
	VIM-P	FAM-CTTCTATCCTGGTGCTGCGCATTCG-BHQ-1		720–744
blaVIM-2	VIM2-F	GCGCTTCGGTCCAGTAGAAC	AF191564	1810–1829	80
	VIM2-R	CTCGCAGACGGGACGTACA		1890–1872
	VIM-P	Same probe as for blaVIM-1		1831–1855
blaIMP-1	IMP1-F	GGGCGGAATAGAGTGGCTTA	AY168635	303–322	93
	IMP1-R	GGCTTGAACCTTACCGTCTTTTT		396–374
	IMP1-P	HEX-CGATCTATCCCCACGTATGCATCTGAATTAACA-BHQ-1		328–360
blaNDM-1	NDM1-F	CGGCATCACCGAGATTGC	FN396876	2547–2564	73
	NDM1-R	CACCGACATCGCTTTTGGT		2620–2602
	NDM1-P	FAM-GCGACTTGGCCTTGCTGTCCTTG-BHQ-1		2568–2590

^aHEX, hexachlorofluorescein; BHQ-1, black hole quencher 1; FAM, 6-carboxyfluorescein.