Table 2.
Species and strain M no. | Date (yr or mo-yr) | First sample type | Ward | repPCR type | PFGE type | Integron type | Genetic location | Plasmid size(s) (kb) |
---|---|---|---|---|---|---|---|---|
Pseudomonas aeruginosa | ||||||||
0 | 2002 | Respiratory tract | SICU | PSA-1 | PSA-A | In77 | C/P | 22 |
19 | Oct-07 | Wound | SW1 | PSA-4 | PSA-B | In853 | C | |
1 | Dec-07 | Respiratory tract | MICU2 | PSA-5 | PSA-C | In583* | C | |
2 | Jan-08 | Stool | MW3 | PSA-6 | PSA-D | In851* | C | |
4 | Apr-08 | Urine | MW2 | PSA-1 | PSA-A | In77* | C | |
3 | Apr-08 | Respiratory tract | SICU | PSA-1 | PSA-A | In77 | C | |
5 | Jun-08 | Respiratory tract | SICU | PSA-1 | PSA-A | In77 | C | |
7 | Sep-08 | Respiratory tract | MICU1 | PSA-2 | PSA-E | In770 | C | |
8 | Sep-08 | Respiratory tract | MICU1 | PSA-2 | PSA-A | In770* | C | |
10 | Oct-08 | Stool | MICU1 | PSA-2 | PSA-F | In770 | C | |
16 | Feb-10 | Respiratory tract | SICU | PSA-1 | ND | In77 | C | |
25 | Feb-10 | Urine | MW2 | PSA-1 | PSA-A | In77 | C | |
28 | May-10 | Urine | MOP | PSA-7 | PSA-G | In770 | ND | |
29 | May-10 | Respiratory tract | SICU | PSA-8 | PSA-H | In851 | C | |
45 | Nov-10 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
46 | Dec-10 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
47 | Dec-10 | Urine | MW1 | PSA-1 | PSA-A | In77b | C | |
48 | Mar-11 | Wound | SICU | PSA-1 | PSA-A | In77b | C | |
57 | Apr-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
49 | May-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
50 | Jun-11 | Urine | SICU | PSA-1 | PSA-A | In77b | C | |
51 | Jun-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
53 | Jun-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
52 | Jun-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
55 | Jul-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
58 | Sep-11 | Wound | SICU | PSA-1 | PSA-A | In77b | C and P | 25 |
63 | Dec-11 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b* | C | |
64 | Jan-12 | Wound | SICU | PSA-1 | PSA-A | In77b | C | |
67 | Mar-12 | Wound | SICU | PSA-1 | PSA-A | In77b | C | |
68 | Mar-12 | Urine | MW1 | PSA-3 | PSA-A | In770 | C | |
69 | Apr-12 | Wound | SICU | PSA-1 | PSA-A | In851 | C | |
70 | May-12 | Respiratory tract | SICU | PSA-1 | PSA-A | In77b | C | |
71 | May-12 | Respiratory tract | MW4 | PSA-3 | PSA-D | In851 | C | |
Enterobacter cloacae | ||||||||
14 | Jun-09 | Urine | MW4 | ENT-1 | ND | In770 | C and two P | 25 and 220 |
15 | Jan-10 | Blood culture | MICU1 | ENT-1 | ND | In770* | C and two P | 25 and 220 |
21 | Mar-10 | Wound | MW2 | ENT-2 | ND | In770 | C and two P | 25 and 220 |
35 | Jul-10 | Urine | MW3 | ENT-2 | ND | In770 | C and two P | 25 and 220 |
44 | Aug-10 | Urine | MW3 | ENT-2 | ND | In770 | C and two P | 25 and 220 |
60 | Dec-11 | Wound | MW3 | ENT-1 | ND | In770 | C and two P | 25 and 220 |
65 | Dec-11 | Urine | MW3 | ENT-2 | ND | In770 | C and two P | 25 and 220 |
Pseudomonas putida | ||||||||
18 | Sep-07 | Urine | MW3 | PUT-3 | ND | In770* | C | |
6 | Sep-08 | Stool | MW3 | PUT-1 | ND | In853* | C | |
12 | Dec-08 | Urine | MW3 | PUT-1 | ND | In853 | C | |
37 | Aug-10 | Urine | MW3 | PUT-2 | ND | In853 | C | |
38 | Aug-10 | Urine | MW3 | PUT-2 | ND | In853 | C | |
Serratia marcescens | ||||||||
9 | Sep-08 | Urine | MICU2 | ND | SMA-A | In770* | P | 140 |
11 | Nov-08 | Urine | SW2 | ND | SMA-B | In770 | C | |
Escherichia coli 17 | Mar-07 | Blood culture | MW5 | ND | ND | In852* | C and two P | 50 and 130 |
Klebsiella oxytoca 43 | Oct-10 | Urine | MW3 | ND | ND | In770* | C | |
Citrobacter freundii 56 | Jul-11 | Wound | SICU | ND | ND | In851* | C |
MBL-positive isolates were given consecutive numbers with the prefix M. DiversiLab repPCR cluster and PFGE types were given numbers and letters, respectively, with an acronym of the species. M0 (73-5671) was collected in 2002 and blaGIM-1 was described by Castanheira et al. (5) on a 22-kb plasmid, while we detected it chromosomally. *, integron sequences were published in GenBank (JX566704 to JX566715) and given INTEGRALL numbers as shown (In77b being a variant of In77). SICU, surgical intensive care unit; MICU, medical intensive care unit; SW, surgical ward; MW, medical ward (numbers indicate different wards); ND, not determined (M28 and M16 strains lost for further analysis); C, chromosome; P, plasmid.