Fig 2.
Susceptibility of NTR-overexpressing LdBOB promastigotes to nifurtimox, (R)-PA-824, and (S)-PA-824. EC50s were determined for nifurtimox (A), (S)-PA-824 (B), and (R)-PA-824 (C) against WT (open circles) and NTR-overexpressing (closed circles) parasites. EC50s of 5.6 ± 0.16 and 0.2 ± 0.09 μM for nifurtimox, 0.96 ± 0.02 and 0.8 ± 0.06 μM for (S)-PA-824, and 0.16 ± 0.003 and 0.11 ± 0.005 μM for (R)-PA-824 were determined against WT and NTR-overexpressing cell lines, respectively. Data are the means of triplicate measurements.