Culture fluorescence due to metX expression and pSenOAS3 in derivatives of strain KK42. (A) Growth of strain KK42 pCLTON2 ± 150 ng ml−1 anhydrotetracycline (atc). (B) Fluorescence of cultures shown in panel A. (C) Growth of strain KK42 pCLTON2 pSenOAS3 at different atc concentrations. (D) Fluorescence of cultures shown in panel C due to increasing atc concentrations. The concentrations (ng ml−1) used are indicated as follows: 0, □; 10, ○; 25, △; 50, ▽; 100, ◇; 150, ▲.