Table 1.
Strains and plasmids used
| Strain or plasmid | Feature(s) or usea | Source or reference |
|---|---|---|
| C. glutamicum strains | ||
| ATCC 13032 | Wild type (WT) of C. glutamicum | ATCC |
| D | WT ΔsdaA ΔpabABC | 19 |
| Ser4 | WT ΔsdaA ΔpabABC (pserA [FBR]-serB-serC); l-serine producer | 19 |
| Cys3 | Ser4ΔaecD; l-cysteine producer | This study |
| KK42 | MH20-22B lysC (FBR) ΔleuCD hom (FBR) | 20 |
| Plasmids | ||
| pJC1 | Shuttle vector, Kmr | 21 |
| pSenLys | Lysine sensor; encodes transcriptional regulator LysG and its target promoter of lysE with a transcriptional fusion to eyfP | 22 |
| pSenOAS1 | Derived from pSenLys; encodes transcriptional regulator CysR and its target promoter of NCgl1289 with a transcriptional fusion to eyfP | This study |
| pSenOAS3 | Derived from pSenLys; encodes CysR and its target promoter of cysI with a transcriptional fusion to eyfP | This study |
| pCLTON1 | Shuttle vector, Kanr | 23 |
| pCLTON2 | Shuttle vector, Spcr | This study |
| pCLTON2metX | pCLTON2 with metX of C. glutamicum | This study |
| pCR007d | pK18mobsacB carrying aecD deletion; Kmr | 8 |
a
Kmr, kanamycin resistance; Spcr, spectinomycin resistance; FBR, feedback-resistant enzyme.