Fig 3.

Complementation of E. coli JW0665-1 (nagE) by genes cbe4532 and cbe4533. A transformant containing recombinant pUC18 carrying cbe4532 and cbe4533 was compared with the control strain transformed with pUC18 alone. (A) Fermentation of GlcNAc on MacConkey agar. (B) Utilization of GlcNAc in cultures growing on LB broth. ■, growth of control; ●, growth of transformant; □, broth GlcNAc concentration of control; ○, broth GlcNAc concentration of transformant. (C) GlcNAc PTS activity in cell extracts. ■, transformant uninhibited; □, control uninhibited; ●, transformant in the presence of 10 mM glucose. The values shown in panels B and C are the average of duplicate experiments.