Fig 5.

Inducible gene silencing of the GFP gene using the MoCel7C promoter in Magnaporthe oryzae. (A) Scatter plots of GFP fluorescence measurements of M. oryzae transformants grown on rich medium versus CMC medium. The GFP silencing construct, ipSilent-GFP, which produces GFP hairpin RNA under the control of the MoCel7C promoter, was introduced into a GFP-expressing strain of M. oryzae. One hundred seven transformants were randomly picked, and their GFP fluorescence was measured 60 h after incubation on the two media. (B) Time course expression profile of the GFP gene in three transformants with ipSilent-GFP. GFP fluorescence of fungal colonies was measured at 24-h intervals for 3 days after transfer to medium containing 1% CMC. The experiment was performed with two biological replicates and two technical replicates. Error bars represent standard deviations.