Fig 3.

Three specific mutations identified in the oleic acid-producing mutants. The locations of mutations fasR20, fasA63^up, and fasA2623 are indicated by dotted lines. The order in which these mutations arose is shown by circled numbers 1 to 3. The fasR20 mutation is located at nucleotide position 59 in the fasR gene (gray gene). The fasA63^up mutation is located 63 bp upstream of the fasA gene. The nucleotide sequence of its surrounding region is also shown. The fasA63^up mutation is indicated by the letter larger than its neighbors. The FasR-biding site fasO is boxed (28). The −10 and −35 regions of a potential promoter of fasA are underlined, and the transcriptional start site is also indicated by a bold and underlined letter (28). Hatched boxes (boxes A to G) along the fasA gene represent nucleotide regions for putative catalytic domains involving in fatty acid synthesis (29, 48). The white part of box G represents a region for a motif sequence (PROSITE motif PS00606) for a 3-ketoacyl-ACP synthase active site. The fasA2623 mutation is located within the motif. Box A represents a region for acetyl-CoA transferase, box B represents a region for enoyl-ACP reductase, box C represents a region for 3-ketoacyl-ACP dehydratase, box D represents a region for malonyl/palmitoyl transferase, box E represents a region for a substrate binding site of ACP, box F represents a region for 3-ketoacyl-ACP reductase, and box G represents a region for 3-ketoacyl-ACP synthase. The genes whose expression is thought to depend on FasR (28) are black.