Fig 4.
Reconstitution of defined mutations in the wild-type genome and its effect on oleic acid production. Wild-type ATCC 13032 carrying the mutations fasR20, fasA63up, fasA2623, and ΔfasR separately or in combination were examined for the ability to produce oleic acid by using the same agar piece assay as in Fig. 2. The images show one result representative of three independent experiments. Plus and minus signs represent the presence and absence of the corresponding mutation in the wild-type background, respectively. The ΔfasR mutant strain carries no other mutation, except for the deletion of the fasR gene.