Table 1.
Lipid production by wild-type ATCC 13032 and strain PCC-6a
| Lipid | Wild type |
Strain PCC-6 |
||
|---|---|---|---|---|
| Production (mg/liter) | Wt % | Production (mg/liter) | Wt % | |
| Free fatty acids | ||||
| C15:1 | 1.61 ± 0.04 | 50.00 ± 0.16 | 2.93 ± 0.06 | 1.05 ± 0.02 |
| C16:0 | —b | — | 46.93 ± 2.03 | 16.76 ± 0.22 |
| C16:1 | 0.71 ± 0.04 | 21.95 ± 0.68 | 6.39 ± 0.21 | 2.28 ± 0.00 |
| C18:0 | — | — | 12.35 ± 0.46 | 4.41 ± 0.03 |
| C18:1 | 0.90 ± 0.01 | 28.06 ± 0.84 | 208.10 ± 5.67 | 74.34 ± 0.23 |
| C20:0 | — | — | 2.50 ± 0.06 | 0.89 ± 0.11 |
| C20:1 | — | — | 0.77 ± 0.03 | 0.28 ± 0.00 |
| Total | 3.21 ± 0.06 | 100.00 ± 0.00 | 279.95 ± 8.50 | 100.00 ± 0.00 |
| Phospholipids | ||||
| DPG | 9.76 ± 0.47 | 100.00 ± 0.00 | 43.18 ± 1.84 | 100.00 ± 0.00 |
| Total | 9.76 ± 0.47 | 100.00 ± 0.00 | 43.18 ± 1.84 | 100.00 ± 0.00 |
a
Culture supernatants were prepared at the points indicated by the arrows in Fig. 6 and then subjected to lipid analysis. The amounts of lipids were determined by using two independent cultures performed as described in the legend to Fig. 6. Values are means ± standard deviations. DPG is diphosphatidylglycerol. Other phospholipids (e.g., phosphatidylinositol, phosphatidylglycerol, and phosphatidic acid) were not detected in either strain.
b
—, not detected.