Fig 6.

Overproduction of STLs by overexpression of the STL-producing genes tlsA and fda. STLs were produced under the resting-cell conditions and then extracted by ethyl acetate. The quantification of STLs was performed using the anthrone-sulfuric acid method, and trehalose was used for the standard curve. STL production nearly doubled with overexpression of tlsA compared to the level of production by the nonoverexpressing strain. The results are averages based on three or more independent determinations, and the standard errors are indicated.