Rhodococcus sp. strains |
|
|
SD-74 |
Wild type |
This study |
330 |
STL productivity-deficient Tn mutant |
This study |
1510 |
STL productivity-deficient Tn mutant |
This study |
1608 |
STL productivity-deficient Tn mutant |
This study |
E. coli strains |
|
|
DH5α |
F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK+ mK+) supE44 λ−
thi-1 gyrA relA1
|
TaKaRa |
JM109 |
recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+
lacIq
lacZΔM15] |
TaKaRa |
BL21(DE3) |
E. coli B, F−
dcm ompT hsdSB(rB+ mB+) gal (DE3) |
TaKaRa |
Plasmids |
|
|
pBluescript II SK(+) |
Cloning vector, Ampr
|
Stratagene |
pSMT3 |
Rhodococcus-E. coli shuttle vector, Hgr |
19 |
pSMT3-tlsA |
pSMST3 derivative carrying traT
|
This study |
pSMT3-fda |
pSMST3 derivative carrying fda
|
This study |
pSMT3-alkB |
pSMST3 derivative carrying alkB
|
This study |
pSMT3-traT-D191A |
pSMT3 derivative carrying mutant tlsA(D191A) |
This study |
pSMT3-traT-H192A |
pSMT3 derivative carrying mutant tlsA(H192A) |
This study |
pSMT3-traT-D196A |
pSMT3 derivative carrying mutant tlsA(D196A) |
This study |
pSTM3-PalkB |
pSMT3 derivative carrying alkB promoter |
This study |
pSMT3-PalkB::tlsA |
pSMT3 derivative for traT overexpression |
This study |
pSMT3-PalkB::fda |
pSMT3 derivative for fda overexpression |
This study |
pET21b |
Apr, His tag, T7 promoter |
Novagen |
pET21b-fda |
pET21b derivative, Fda expression vector |
This study |
pET21b-fda-His |
pET21b derivative, Fda-His expression vector |
This study |