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. 2013 Nov;79(22):7082–7090. doi: 10.1128/AEM.01664-13

Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmid Relevant properties Source or reference
Rhodococcus sp. strains
    SD-74 Wild type This study
    330 STL productivity-deficient Tn mutant This study
    1510 STL productivity-deficient Tn mutant This study
    1608 STL productivity-deficient Tn mutant This study
E. coli strains
    DH5α F ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK+ mK+) supE44 λ thi-1 gyrA relA1 TaKaRa
    JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+ lacIq lacZΔM15] TaKaRa
    BL21(DE3) E. coli B, F dcm ompT hsdSB(rB+ mB+) gal (DE3) TaKaRa
Plasmids
    pBluescript II SK(+) Cloning vector, Ampr Stratagene
    pSMT3 Rhodococcus-E. coli shuttle vector, Hgr 19
    pSMT3-tlsA pSMST3 derivative carrying traT This study
    pSMT3-fda pSMST3 derivative carrying fda This study
    pSMT3-alkB pSMST3 derivative carrying alkB This study
    pSMT3-traT-D191A pSMT3 derivative carrying mutant tlsA(D191A) This study
    pSMT3-traT-H192A pSMT3 derivative carrying mutant tlsA(H192A) This study
    pSMT3-traT-D196A pSMT3 derivative carrying mutant tlsA(D196A) This study
    pSTM3-PalkB pSMT3 derivative carrying alkB promoter This study
    pSMT3-PalkB::tlsA pSMT3 derivative for traT overexpression This study
    pSMT3-PalkB::fda pSMT3 derivative for fda overexpression This study
    pET21b Apr, His tag, T7 promoter Novagen
    pET21b-fda pET21b derivative, Fda expression vector This study
    pET21b-fda-His pET21b derivative, Fda-His expression vector This study