. 2013 Nov;79(22):7082–7090. doi: 10.1128/AEM.01664-13

Table 2.

Amino acid alignments of selected alkane monooxygenases

Protein^a	1st residue	Sequence (1st, 2nd, or 3rd segment)^b	Last residue
`AlkB1-Q15`	`131`	`GVTAGIGINTAHELGHKKESVERWLSKIVLAQSAYGHFYLEHNRGHHVRVSTPEDPATSRFGETLYGFWP`	`198`
`AlkB2-Q15`	`147`	`GCVAGIGINTAHELGHKKDDLERWLSKITLAQSFYGHFYIEHNRGHHVRVATPEDPASSRFGESFWTFLP`	`216`
`AlkB1-16531`	`131`	`GVTAGIGINTAHELGHKKESVERWLSKIVLAQSAYGHFYLEHNRGHHVRVSTPEDPATSRFGETLYGFWP`	`198`
`AlkB2-16531`	`147`	`GCVAGIGINTAHELGHKKDDLERWLSKITLAQSFYGHFYIEHNRGHHVRVATPEDPASSRFGESFWTFLP`	`216`
`AlkB-H37Rv`	`150`	`GVLGGVGINTAHEMGHKKDSLERWLSKITLAQTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLP`	`219`
`AlkM-ADP1`	`143`	`GAINGIAVNTAHELSHKADRLDHILSHLALVPTGYNHFRIEHPYGHHKRAATPEDPASSQMGETFYEFWP`	`208`
`AlkB-GPo1`	`136`	`GIVNGLALNTGHELGHKKETFDRWMAKIVLAVVGYGHFFIEHNKGHHRDVATPMDPATSRMGESIYKFSI`	`196`
`AlkB-SD74`	`131`	`GVTAGIGINTAHELGHKKENLERWLSKIVLAQSAYGHFYIEHNRGHHVRVSTPEDPASSRFGESFYRFWP`	`198`
`AlkB1-Q15`	`199`	`RSVGGGLKSAWHLEKTRFERLGTTHWSIKN----DVLNAWLMSVVLFGVALAVFGIGIAPYLVIQAFIGF`	`264`
`AlkB2-Q15`	`217`	`RSVWGSLRSSWSLEKARLDRLGKKPWT---I-RNGVLHSWLMSVVLFGVLVAVFGLSVLPFLVLQAVFGF`	`282`
`AlkB1-16531`	`199`	`RSVGGGLKSAWHLEKTRFERLGTTHWSIKN----DVLNAWLMSVVLFGVALAVFGIGIAPYLVIQAFIGF`	`264`
`AlkB2-16531`	`217`	`RSVWGSLRSSWSLEKARLDRLGKKPWT---I-RNDVLHSWLMSVVLFGVLVAVFGLSVLPFLVLQAVFGF`	`282`
`AlkB-H37Rv`	`220`	`RSVIGGLRSAVHLEAQRLRRLGVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGF`	`289`
`AlkM-ADP1`	`209`	`RTVFGSLKSAIEIETHRLKRKGKKFWSKDNEL----LQGWGMSAAFHSSIIAIFGKGTIPYLVTQAFYGI`	`274`
`AlkB-GPo1`	`197`	`REIPGAFIRAWGLEEQRLSRRGQSVWSFDNEILQPMI----ITVILYAVLLALFGPKMLVFLPIQMAFGW`	`262`
`AlkB-SD74`	`199`	`RSVAGGLKSAWHLEKARFERLGTTHWSIKN----DVLNAWLMTLVLFGAVVAAFGIGILPYVLIQAVIGF`	`264`
`AlkB1-Q15`	`265`	`SLLEAINYLEHYGLLRQKTASGRYERCTPAHSWNSDRICTNVFLYHLQRHSDHHANPTRRYQALRSMEEA`	`334`
`AlkB2-Q15`	`283`	`CLLETVNYLEHYGLKRRRLDSGRYERAAPEHSWNSDHICTNIFLYHLQRHSDHHANPTRRYQTLRSMDGA`	`352`
`AlkB1-16531`	`265`	`SLLEAINYLEHYGLLRQKTASGRYERCTPAHSWNSDRICTNVFLYHLQRHSDHHANPTRRYQALRSMEEA`	`334`
`AlkB2-16531`	`283`	`CLLETVNYLEHYGLKRRRLDSGRYERAAPEHSWNSDHICTNIFLYHLQRHSDHHANPTRRYQTLRSMDGA`	`352`
`AlkB-H37Rv`	`290`	`SLLEAVNYLEHYGLLRQKSANGRYERCAPVHSWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGA`	`359`
`AlkM-ADP1`	`275`	`SLFEIINYIEHYGLKRQKRADGNYERTMPEHSWNNNNIVTNLFLYQLQRHSDHHAYPTRPFQALRHFDEA`	`344`
`AlkB-GPo1`	`263`	`WQLTSANYIEHYGLLRQKMEDGRYEHQKPHHSWNSNHIVSNLVLFHLQRHSDHHAHPTRSYQSLRDFPGL`	`332`
`AlkB-SD74`	`265`	`SLLEAINYLEHYGLRRQKTASGRYERCTPAHSWNSDRLCTNVFLYHLQRHSDHHANPTRRYQTLRSMDQA`	`334`

AlkB-SD74, putative alkane monooxygenase of SD-74; AlkB1-16531 and AlkB2-16531, alkane monooxygenases of Rhodococcus sp. strain Q15 (31); AlkB1-Q15 and AlkB2-Q15, alkane monooxygenases of R. erythropolis NRRLB-16531 (30); AlkB-H37, putative AlkB of M. tuberculosis H37Rv (accession no. NP_217769); Rv AlkM-ADPI, alkane monooxygenase of Acinetobacter sp. ADP1 (accession no. AJ002316); AlkB-GPo1, alkane monooxygenase of Pseudomonas putida GPo1 (accession no. AJ245436).

Underlines indicate conserved Hist-1, Hist-2, and Hist-3 boxes and HYG motifs.