Table 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | General cloning host | Coli Genetic Stock Center |
| E. coli DH5α competent cells | Subcloning efficiency competent cells | Invitrogen |
| E. coli MG1655 | Escherichia coli λ− F− rph-1 | Coli Genetic Stock Center |
| E. coli BL21(DE3) | Escherichia coli F− λ(DE3 [lacI lacUV5-T7]) | Coli Genetic Stock Center |
| B. bifidum | Bifidobacterium bifidum (Tissier 1900) ATCC 29521 | BCCM/LMG |
| sMEMO_WT | E. coli MG1655 ΔgalETKM ΔgalU ΔushA Δugd Δagp | This study |
| sMEMO_UgpA | E. coli MG1655 ΔgalETKM ΔgalU ΔushA Δugd Δagp carrying pCX-Kan-P22-ugpA | This study |
| sMEMO_GalU | E. coli MG1655 ΔgalETKM ΔgalU ΔushA Δugd Δagp carrying pCX-Kan-P22-galU | This study |
| sMEMO_GalT1 | E. coli MG1655 ΔgalETKM ΔgalU ΔushA Δugd Δagp carrying p10-Trc-galT1 | This study |
| sMEMO_GalT2 | E. coli MG1655 ΔgalETKM ΔgalU ΔushA Δugd Δagp carrying p10-Trc-galT2 | This study |
| sMEMO_His_GalT1 | E. coli BL21(DE3) carrying p10-Trc-His-galT1 | This study |
| sMEMO_His_GalT2 | E. coli BL21(DE3) carrying p10-Trc-His-galT2 | This study |
| Plasmid vectors | ||
| pCX-Kan-P22 | Constitutive expression vector with P22 promoter, Ampr Kanr | 40 |
| pCX-Kan-P22-ugpA | pCX-Kan-P22 vector carrying ugpA from B. bifidum | This study |
| pCX-Kan-P22-galU | pCX-Kan-P22 vector carrying galU from E. coli | This study |
| p10-Trc | Inducible expression vector, Cmr | 41 |
| p10-Trc-galT1 | p10-Trc carrying galT1 from B. bifidum | This study |
| p10-Trc-galT2 | p10-Trc carrying galT2 from B. bifidum | This study |
| p10-Trc-His-galT1 | p10-Trc carrying galT1 from B. bifidum with His6 tag | This study |
| p10-Trc-His-galT2 | p10-Trc carrying galT2 from B. bifidum with His6 tag | This study |
| pKD46 | λ Red recombinase expression, Ampr | 18 |
| pCP20 | FLP recombinase expression, Ampr Cmr | 18 |
| pKD4 | Kan cassette template, Kanr Ampr | 18 |