Table 3.

List of primers used in this study

Primer	Oligonucleotide sequence (5′–3′)a
Fw_galETKM_del	CTGGTGATTTGAACAATATGAGATAAAGCCCTCATGACGAGGGCGTAACAGTGTAGGCTGGAGCTGCTTC
Rv_galETKM_del	CTTTGTTATGCTATGGTTATTTCATACCATAAGCCTAATGGAGCGAATTATGCATATGAATATCCTCCTTAG
Fw_galU_del	CGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTT
Rv_galU_del	GCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGC
Fw_ushA_del	TCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTGTGTAGGCTGGAGCTGCTTC
Rv_ushA_del	CCCGCCGCGATTAAGCATTGTGCCGGATGCAAACATCCGGCACTTTCGGACATATGAATATCCTCCTTAG
Fw_ugd_del	TGTAAGTAACAAAAGACAATCAGGGCGTAAATAGCCCTGATAACAAGATGGTGTAGGCTGGAGCTGCTTC
Rv_ugd_del	GATGCTAAAAACATCATGATTCACAGTTAAGTTAATTCTGAGAGCATGAACATATGAATATCCTCCTTAG
Fw_agp_del	CATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAGTGTAGGCTGGAGCTGCTTC
Rv_agp_del	TAAAAACGTTTAACCAGCGACTCCCCCGCTTCTCGCGGGGGAGTTTTCTGCATATGAATATCCTCCTTAG
Fw_galU_EcoRI	CCGGCGAATTCGGAGGAAACAAAGATGGCTGCCATTAATACGAAAG
Rv_galU_SacI	CGCCGAGCTCTTACTTCTTAATGCCCATCTC
Fw_ugpA_EcoRI	CCGGCGAATTCGGAGGAAACAAAGATGTTTGCCGAAGATCTGAAACG
Rv_ugpA_SacI	CGCCGAGCTCTCACACCCAATCACCGGGCTCGATG
Fw_galT1_NdeI	AGGTCGCGCATATGGCAGAAATCACCAACTACAC
Rv_galT1_PmeI	TATGTTTAAACGCTAGCCTCGAGGCGGCCGCGAGCTCTAAAGGGCCGGCCTAAACGAATTCTCAGTCGGAGATGTCGATCTG
Fw_galT2_NdeI	AGGTCGCGCATATGACCACGGAAGAGAAGAAGG
Rv_galT2_PmeI	TATGTTTAAACGCTAGCCTCGAGGCGGCCGCGAGCTCTAAAGGGCCGGCCTAAACGAATTCCTAATGCTGAGTATGGAATCCGAGGCCTTCC
Fw_galT1_his	ATGGGCGGCTCACACCACCACCACCACCACGGTATGGCGTCTATGGCAGAAATCACCAACTAC
Fw_galT2_his	ATGGGCGGCTCACACCACCACCACCACCACGGTATGGCGTCTATGACCACGGAAGAGAAGAAG
Rv_galT1_his	CAATGATGATGATGATGATGGTCGACTCAGTCGGAGATGTCGATCTGTC
Rv_galT2_his	CAATGATGATGATGATGATGGTCGACCTAATGCTGAGTATGGAATC
Fw_backbone_p10	AGACGCCATACCGTGGTGGTGGTGGTGGTGTGAGCCGCCCATATGTTATTCCTCCTTATTTAATC
Rv_backbone_p10	GTCGACCATCATCATCATCATCATTG

^aRestriction sites are underlined. His₆ tags are underlined and in bold.