Table 4.
Kinetic parameters for UgpA and GalU crude protein extractsa
| Protein extract | Sp act (U mg−1) |
Km (mM) |
|
|---|---|---|---|
| H1P | UTP | ||
| UgpA (G1P) | 13.0 ± 0.08 | 0.098 ± 0.011 | 0.042 ± 0.006 |
| UgpA (gal1P) | 0.40 ± 0.01 | 0.148 ± 0.034 | 0.032 ± 0.010 |
| GalU (G1P) | 1.88 ± 0.03 | 0.010b | 0.070b |
| GalU (gal1P) | ND | ||
a
Reactions were carried out at pH 6.5 and 37°C. ND, not detected. Experiments were carried out in triplicate, and standard deviations are shown.
b
Data from the work of Weissborn et al. (27). Reaction carried out at pH 7.8 and 37°C.