Table 4.
Plate counts and quantitative PCR analysis of S. enterica serovar Enteritidis PT21 in artificially contaminated PBS and chicken homogenate samplesa
| Sample type | Sample | Plate count (log CFU/ml) (mean ± SEM) | qPCR (log GCE/mlb) (mean ± SE) |
|---|---|---|---|
| PBS (n = 4) | Unspiked sample | UDc | UD |
| Artificially spiked sample | 2.68 ± 0.09 | 2.53 ± 0.08 | |
| Concentrated sample | 4.92 ± 0.15 | 5.55 ± 0.06 | |
| Chicken homogenates (n = 8) | Unspiked sample | UD | UD |
| Artificially spiked sample | 3.26 ± 0.30 | 2.57 ± 0.13 | |
| Concentrated sample | 4.95 ± 0.54 | 5.57 ± 0.09 |
a
Replicate samples were artificially contaminated, and the experiment was repeated four times.
b
GCE, genomic cell equivalents.
c
UD, undetermined.