Table 2.
Genotypic and phenotypic characteristics of O26 isolates
| Strain | Ora | H type | Virulence marker(s) |
Adhesionb | FPd |
PGe | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| O island |
Adhesin | Virulence plasmid | Additional | S/RF | RH/D | ||||||
| 71 | 122 | ||||||||||
| EHEC derivatives | |||||||||||
| 1.84 | DC | H8 | nleF, nleA | eae, efa2, iha | irp2, fyuA | NA | +/+ | +/+ | B1 | ||
| 2361-4/85 | DC | H8 | nleF, nleA | eae, efa2 (lif), iha | espV, irp2, fyuA | DA | +/+ | +/− | B1 | ||
| 234-1 CII | DC | H8 | nleF, nleA | eae, efa1, efa2 (lif), iha | irp2, fyuA | LAL | +/+ | −/+ | B1 | ||
| 32Fe/2 vera | DC | H8 | nleF, nleA | eae, efa2, iha | astA, espV, irp2, fyuA | LAL | +/+ | +/+ | B1 | ||
| 1-84/RJ | DC | H8 | nleF, nleA | eae, efa1, efa2 (lif), iha | espV, irp2, fyuA | NA | +/+ | +/− | B1 | ||
| 3561-2 | DC | H8 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae, efa1, efa2, iha | espV, irp2, fyuA | DA | +/+ | +/+ | B1 | |
| 54/82 HC | DC | H8 | nleF, nleA | eae, efa2, iha | espV, irp2, fyuA | LAL | +/+ | +/− | B1 | ||
| 14-1 CII | CC | H8 | nleF, nleA | eae, efa1, efa2 (lif), iha | irp2, fyuA | LA | +/+ | +/+ | B1 | ||
| 3282-7 | CC | H8 | nleF | eae-beta | terE, astA, espV | NA | +/+ | +/+ | B1 | ||
| 387-8 CII | DC | H8 | nleF, nleH1-2 | eae-beta, iha | terE, espV | LAL | +/+ | +/− | B1 | ||
| 200/82 HSP | DC | H8 | nleF, nleH1-2 | eae-beta | terE, nleD-2, astA, espV | DA | +/+ | +/+ | B1 | ||
| 3280-7/86 | CC | H8 | nleF | eae-beta, lpfAO113 | terE, nleD-2, espV | NA | +/+ | +/+ | B1 | ||
| O26 TR EPM | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa, lpfAO113, toxB | ehxA, espP, katP | ureD, terE, hlyA, irp2, fyuA | LAL | +/+ | −/− | B1 |
| 0791-1/85c | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa, lpfAO113 | espP | ureD, terE, irp2, fyuA | NA | +/+ | −/− | B1 |
| 1971-1/85c | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa, ldaH | ureD, terE, irp2, fyuA | LA | +/+ | +/+ | B1 | |
| 72-B/87 | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa | espI | ureD, terE, irp2, fyuA | LA | +/+ | +/− | B1 |
| 3561-2/86 | DC | H11 | nleF, nleA | eae-beta, efa1, efa2 (lif), iha, paa, lpfAO113 | ureD, terE, irp2, fyuA | DA | +/+ | +/− | B1 | ||
| 1551-3/85c | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa | ureD, terE, irp2, fyuA | DA | +/+ | +/+ | B1 | |
| 7-81 HSJ | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa | ureD, terE, irp2, fyuA | LAL | +/+ | +/+ | B1 | |
| 3451-3/86 | DC | H11 | nleF, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa | ehxA, espP, espI | ureD, terE, hlyA, irp2, fyuA | NA | +/+ | −/− | B1 |
| 2911-2/84 | DC | H11 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa, toxB | ehxA, espP | ureD, terE, astA, irp2, fyuA | NA | +/+ | +/+ | B1 | |
| 93-81 HSP | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, toxB | ehxA, espP, espI | ureD, terE, hlyA, irp2, fyuA | LA/DA | +/+ | +/+ | B1 |
| 2012-1 (2) | CC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), iha, paa, toxB, ldaH | ehxA, espP | ureD, terE, astA, irp2, fyuA | LAL | +/+ | +/+ | B1 |
| aEPEC | |||||||||||
| 4851-3/86 | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa, toxB, ldaH | terE, irp2, fyuA | LAL | +/+ | +/+ | B1 | |
| H2 | DC | H11 | nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif) | irp2, fyuA | LA | +/+ | +/+ | B1 | |
| 2271-1/85 | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa, ldaH | terE, irp2, fyuA | LAL | +/+ | +/+ | B1 | |
| 25623 | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa | terE, astA, irp2, fyuA | LAL | +/+ | +/+ | B1 | |
| 277-2 CII | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa | terE, irp2, fyuA | LA | +/+ | +/+ | B1 | |
| 316-10 CII | DC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa | ureD, terE, irp2, fyuA | AA | +/+ | +/+ | B1 | |
| 324-1 CII | CC | H11 | nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 | irp2, fyuA | LA | +/+ | +/+ | A | |
| 2010-1/85 | CC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa, lpfAO113, ldaH | terE, irp2, fyuA | LA | +/+ | +/+ | B1 | |
| 2490-1/85 | CC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa, lpfAO113, ldaH | terE, irp2, fyuA | LA | +/+ | +/+ | B1 | |
| 2492-1 | CC | H11 | nleF, nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), paa, ldaH | terE, astA, irp2, fyuA | LAL | +/+ | +/+ | B1 | |
| 1C50 | MM | H11 | nleA, nleH1-2 | nleB, ent, nleE | eae-beta, efa1, efa2 (lif), lpfAO113 | irp2, fyuA | DA | +/+ | +/+ | B1 | |
| Avirulent | |||||||||||
| 268-10 CII | CC | H32 | NA | −/− | +/+ | A | |||||
| 17354-2/84 | NTW | H32 | ldaH | espI | NA | −/− | +/+ | A | |||
| 2C27 | MM | H32 | NA | −/− | +/+ | A | |||||
| 3C21 | MM | H32 | NA | −/− | +/+ | A | |||||
| 4C2 | MM | H32 | NA | −/− | +/+ | A | |||||
| 4C27 | MM | H32 | NA | −/− | +/+ | A | |||||
| 6K10 | MM | H32 | NA | −/− | +/+ | A | |||||
| 6K60 | MM | H32 | nleB | NA | −/− | +/+ | A | ||||
| 6C41 | MM | H32 | NA | −/− | +/+ | A | |||||
| 1K10 | MM | H32 | NA | −/− | +/+ | A | |||||
a
Or, strain origin; DC, diarrheic child; CC, control child; MM, minced meat; NTW, nontreated water.
b
Adhesion patterns on HeLa cells. LA, localized adherence; LAL, localized adherence-like; AA, aggregative adherence; DA, diffuse adherence; NA, nonadherent.
c
Isolate analyzed by its host cell interactions and TEM.
d
FP, fermentation pattern; S/RF, sucrose/raffinose; R/D, rhamnose/dulcitol; +, positive reaction; −, negative reaction.
e
PG, phylogenetic group.