Table 1.
ORFs in the thioviridamide biosynthetic gene clustera
| Protein | Size (aa) | Homologous protein (origin) | Identity/similarity (%) | GenBank or NCBI accession no. | Deduced function |
|---|---|---|---|---|---|
| Orf1 | 401 | DNA polymerase III subunit delta′ (S. davawensis) | 90/93 | CCK28488 | |
| Orf2 | 529 | Protease (S. avermitilis) | 83/87 | NP_825801 | |
| TvaA | 75 | ABC transporter (Penicillium griseofulvum) | 46/62 | ACR02669 | Precursor peptide |
| TvaB | 275 | SARP family regulator (S. lavendulae) | 35/55 | BAG74713 | Regulator |
| TvaC | 377 | Phosphotransferase family protein (Nostoc sp.) | 29/45 | YP_007077015 | Unknown |
| TvaD | 328 | Hypothetical protein (Calothrix sp.) | 25/41 | YP_007137464 | Unknown |
| TvaE | 308 | Aminoglycoside phosphotransferase (Frankia sp.) | 48/65 | EHI90509.1 | Unknown |
| TvaF | 197 | Phosphopantothenoylcysteine decarboxylase (Exiguobacterium sp.) | 39/60 | YP_002887224 | Oxidative decarboxylase |
| TvaG | 407 | Type 12 methyltransferase (Cyanothece sp.) | 43/61 | YP_002481771 | Methyltransferase |
| TvaH | 452 | Methanogenesis marker protein 1 (Methanolinea tarda) | 36/53 | EHF09819.1 | Unknown |
| TvaI | 218 | TfuA-like core domain-containing protein (M. tarda) | 44/63 | EHF09820.1 | Regulator |
| TvaJ | 280 | Phytanoyl-CoA dioxygenase (Roseobacter litoralis) | 29/44 | YP_004691882 | Oxygenase |
| TvaK | 220 | Papain family cysteine protease (Tannerella forsythia) | 27/39 | YP_005014309 | Protease |
| TvaL | 282 | Integral membrane protein (S. pristinaespiralis) | 31/49 | EDY64257.2 | Unknown |
| TvaM | 160 | Histidine kinase (S. somaliensis) | 55/66 | WP_010468209.1 | Regulator |
| TvaN | 1,003 | Large transcriptional regulator (S. pristinaespiralis) | 67/75 | EDY63194.2 | Regulator |
| TvaO | 94 | Hypothetical protein (S. viridochromogenes) | 54/68 | ELS50220.1 | Unknown |
| Orf3 | 1,089 | Transcriptional regulator (S. avermitilis) | 63/69 | NP_825812 | |
| Orf4 | 561 | DNA polymerase I (S. griseoflavus) | 87/92 | EFL40026.1 |
a
S., Streptomyces; SARP, Streptomyces antibiotic regulatory protein; CoA, coenzyme A.