Table 2.
Detection and quantification of rdhA genes during PCE dechlorination in SL2 consortiaa
| Consortium | Time (days) | Concn of chloride formed (mM) | Relative abundance determined by: |
|||||
|---|---|---|---|---|---|---|---|---|
| T-RFLP analysis |
qPCR |
|||||||
| pceATCE | pceADCE | rdhA2 | pceATCE | pceADCE | rdhA2 | |||
| SL2-PCEc | 2 | 4.4 ± 0.0 | 57 ± 2 | ND | 44 ± 2 | 41 ± 2 | ND | 59 ± 2 |
| 3 | 8.2 ± 0.3 | 54 ± 0 | ND | 46 ± 0 | 43 ± 1 | ND | 57 ± 1 | |
| 4 | 9.5 ± 0.5 | 53 ± 1 | ND | 48 ± 1 | 36 ± 1 | ND | 64 ± 1 | |
| SL2-TCE | 1 | 0.9 ± 0.0 | ND | 51 ± 3 | 49 ± 3 | ND | 36 ± 2 | 64 ± 2 |
| 2 | 8.6 ± 0.2 | ND | 56 ± 3 | 44 ± 3 | ND | 43 ± 2 | 57 ± 2 | |
| 3 | 9.9 ± 0.3 | ND | 57 ± 1 | 44 ± 1 | ND | 45 ± 4 | 55 ± 4 | |
| 4 | 11.5 ± 0.4 | ND | 58 ± 2 | 43 ± 2 | ND | 44 ± 1 | 56 ± 1 | |
| SL2-PCEb | 3 | 4.0 ± 0.0 | 35 ± 3 | 17 ± 5 | 48 ± 2 | 35 ± 3 | 11 ± 2 | 54 ± 6 |
| 4 | 6.2 ± 0.0 | 39 ± 3 | 12 ± 4 | 49 ± 2 | 35 ± 6 | 7 ± 1 | 58 ± 4 | |
| 5 | 8.7 ± 0.3 | 40 ± 2 | 11 ± 2 | 50 ± 4 | 32 ± 4 | 7 ± 0 | 61 ± 5 | |
| 6 | 10.8 ± 0.4 | 35 ± 1 | 18 ± 1 | 48 ± 1 | 27 ± 2 | 15 ± 2 | 58 ± 2 | |
| 7 | 13.2 ± 0.2 | 29 ± 2 | 27 ± 1 | 44 ± 2 | 22 ± 3 | 27 ± 2 | 51 ± 5 | |
| 8 | 16.0 ± 0.0 | 26 ± 0 | 30 ± 1 | 44 ± 1 | 16 ± 1 | 31 ± 4 | 52 ± 8 | |
| 9 | 18.4 ± 0.0 | 20 ± 3 | 37 ± 6 | 43 ± 8 | 11 ± 1 | 31 ± 6 | 58 ± 5 | |
| 10 | 19.3 ± 0.9 | 22 ± 4 | 35 ± 3 | 44 ± 2 | 10 ± 1 | 35 ± 0 | 55 ± 8 | |
a
Data are means ± standard deviations. ND, not detected.