Table 1.
Infectivity reduction of HAV, MNV, and TV on alfalfa seeds and in HBSS stored at 22°C for up to 50 days
| Virus | Matrix | Infectivity reduction of virus (log PFU/g or log TCID50/g)a on day: |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 2 | 5 | 10 | 15 | 20 | 30 | 50 | ||
| HAV | Seeds | a 1.41 ± 0.19 A | a 1.44 ± 0.32 A | a 2.02 ± 0.93 A | a 1.61 ± 0.09 A | a 1.78 ± 0.14 A | a 2.15 ± 0.39 A | a 3.68 ± 0.00 B | a 3.58 ± 0.21 B |
| HBSS | b 0.00 ± 0.00 A | b 0.05 ± 0.00 A | a 0.80 ± 0.35 B | a 1.80 ± 0.35 C | a 1.80 ± 0.35 C | a 2.38 ± 0.00 CD | a 2.80 ± 0.35 D | a 4.22 ± 0.24 E | |
| MNV | Seeds | a −0.09 ± 0.24 A | a 0.76 ± 0.63 B | a 2.18 ± 0.03 C | a 2.46 ± 0.04 CD | a 2.86 ± 0.14 DE | a 3.14 ± 0.21 EF | a 3.90 ± 0.22 F | a 4.85 ± 0.19 G |
| HBSS | a 0.00 ± 0.00 A | a −0.32 ± 0.43 A | a 2.24 ± 0.11 B | b 2.97 ± 0.03 C | a 3.19 ± 0.10 C | a 3.82 ± 0.14 CD | a 4.22 ± 0.13 D | a 4.94 ± 0.31 E | |
| TV | Seeds | a 0.44 ± 0.07 A | a 0.57 ± 0.12 B | a 0.81 ± 0.04 B | a 1.53 ± 0.08 C | a 2.01 ± 0.17 D | a 2.15 ± 0.16 D | a 2.19 ± 0.09 D | a 2.58 ± 0.21 E |
| HBSS | b 0.00 ± 0.00 A | a 0.03 ± 0.34 A | a 0.77 ± 0.28 B | b 0.91 ± 0.03 BC | b 1.00 ± 0.06 BC | b 1.24 ± 0.03 C | b 1.69 ± 0.01 D | b 1.84 ± 0.08 D | |
a
Values are means ± standard deviations (SD) from three replicates; values in rows with the same preceding letter indicate no significant difference (P > 0.05) between seeds and HBSS for each virus; values in rows with the same following letter indicate no significant difference (P > 0.05) in virus survival between different sampling days.