Table 3.
Changes in the fatty acid and DMA compositions of nonadapted and butanol-adapted cells of strain GK12 in the presence of butanol
| Fatty acid or DMAa | Fatty acid or DMA compositionb (%) of strain GK12 |
||||||
|---|---|---|---|---|---|---|---|
| Nonadapted cells grown with: |
Butanol-adapted cellsc grown with: |
||||||
| No solvents | 2.0% butanol | 2.5% butanol | No solvents | 2.0% butanol | 2.5% butanol | 3.0% butanol | |
| C14:0 | 24.5 ± 1.1 | 6.6 ± 0.5 | 1.5 ± 0.8 | 27.0 ± 1.6 | 26.1 ± 0.4 | 5.2 ± 0.7 | 1.1 ± 0.4 |
| C16:0 | 23.5 ± 1.4 | 23.7 ± 2.2 | 21.6 ± 1.1 | 25.1 ± 0.3 | 27.8 ± 0.6 | 31.2 ± 0.9 | 19.2 ± 0.4 |
| C18:0 | 10.8 ± 0.6 | 30.4 ± 0.7 | 42.1 ± 1.3 | 3.8 ± 0.5 | 5.2 ± 0.5 | 35.2 ± 0.2 | 48.1 ± 0.8 |
| C20:0 | 2.8 ± 0.6 | 10.1 ± 1.3 | 14.1 ± 1.3 | 2.6 ± 0.5 | 4.7 ± 0.5 | 6.6 ± 0.2 | 19.4 ± 0.6 |
| C14:0 DMA | 4.9 ± 1.3 | 0.6 ± 0.2 | ND | 5.5 ± 0.5 | 2.7 ± 1.2 | ND | ND |
| C16:0 DMA | 18.0 ± 1.7 | 1.8 ± 0.5 | 1.6 ± 0.4 | 10.3 ± 1.3 | 4.2 ± 0.3 | 2.1 ± 0.3 | 1.1 ± 0.4 |
| C18:0 DMA | 15.5 ± 0.8 | 26.8 ± 2.2 | 19.1 ± 1.9 | 25.7 ± 0.6 | 29.3 ± 0.5 | 19.6 ± 0.4 | 11.2 ± 0.9 |
| Total C14 | 29.4 ± 1.4 | 7.2 ± 0.4 | 1.5 ± 0.7 | 32.5 ± 1.4 | 28.8 ± 1.1 | 5.2 ± 0.7 | 1.1 ± 0.4 |
| Total C16 | 41.5 ± 1.9 | 25.5 ± 2.0 | 23.3 ± 1.0 | 35.5 ± 1.2 | 32.0 ± 0.6 | 33.3 ± 0.8 | 20.3 ± 0.5 |
| Total C18 | 26.3 ± 0.9 | 57.3 ± 2.0 | 61.2 ± 2.0 | 29.4 ± 0.7 | 34.5 ± 0.6 | 54.8 ± 0.4 | 59.3 ± 1.0 |
a
Abbreviations: CX:Y, fatty acid containing X carbon atoms with Y double bonds; total X, the sum of fatty acids and DMAs with an acyl chain length of X.
b
Each fatty acid composition value is shown as a percentage of whole-cell lipids. Values are means ± standard deviations of three independent measurements. ND, not detected.
c
Cell adaptation was achieved by 15 consecutive passages with 2.0% butanol.