Fig 2.

Phenotypic analysis of N. crassa Δacs strains on glucose. (A) Macroscopic morphology of the WT (FGSC 2489) and Δacs strains. (B) Biomass accumulation in submerged liquid culture in VM–2% glucose. (C) Gas chromatography-flame ionization detection (GC-FID) quantification of fatty acid methyl esters (FAMEs) derived from total lipids during time course in VM–2% glucose. Lipid was quantified per mg of lyophilized biomass. (D) Fatty acid (FA) composition of total cellular lipid of N. crassa Δacs strains. Individual FAMEs derived from total lipids were quantified using GC-FID. Fatty acids are given as a mass fraction of total derivatized FA at the 60-h growth point for all strains, except Δacs-5, which was analyzed at 72 h. Ninety-seven percent of total cellular FAs comprise C16:0, C16:1, C18:0, C18:1, C18:2, and C18:3. (E) FA profile for WT and Δacs-5 strains during early time points. All values are representative of quadruplicate biological samples. Error bars indicate standard deviations.