Fig 6.

Gene complementation of the C9 mutant rescues the wild-type phenotype. (A) The plasmid construct used to complement the attenuated C9 parasite line was developed using the full-length PF3D7_1305500 ORF inserted adjacent to a BSD drug selection cassette. (B) RT-PCR analysis detected PF3D7_1305500 transcripts in NF54 and the complemented parasites (E3 and E8), but not in the C9 MKP-null mutant (upper arrow). As a positive control, 18S RNA was also included for each sample (lower arrow). (C) The relative fold changes of complemented parasites (E3 and E8) were statistically the same as those of wild-type NF54, demonstrating successful rescue of the phenotype.