Fig 1.

CMF22 is broadly conserved among organisms with motile flagella. (A) Representative organisms from each of the indicated eukaryotic clades are shown along with a depiction indicating whether these organisms have a motile flagellum, an immotile flagellum, or no flagellum. The presence or absence of CMF22 orthologues is noted in the right-most column. Only organisms for which the complete genome sequence is available were considered. Groups are based on the work of Keeling et al. (53). (B) The CMF22 domain architecture is conserved in diverse organisms. The schematic shows the predicted domain architecture for CMF22 orthologues from the indicated organisms. The IQ and AAA motifs were identified using SMART (black and gray boxes) or individual alignments (hash-marked box). The organisms (with their GenBank accession numbers in parentheses) used were as follows: Trypanosoma brucei (XP_828418.1), Homo sapiens (NP_001257513.1), Chlamydomonas reinhardtii (XP_001690665.1), Paramecium tetraurelia (XP_001429179.1), and Bigelowiella natans (jgi|Bigna1|142761). aa, amino acids.