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. 2013 Sep;12(9):1202–1213. doi: 10.1128/EC.00068-13

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Fig 2 — CMF22 is associated with the flagellum skeleton. (A) Western blot of total cell lysates from control (2913) or CMF22-HA-tagged (CMF22-HA) cells. Blots were probed with antibodies against the HA epitope or loading control (tubulin). (B) Western blot of whole-cell lysates (lane L) and the indicated subcellular fractions from CMF22-HA cells. Blots were probed with antibodies against the indicated proteins. Fractions correspond to nonionic detergent-soluble (lane S1) and insoluble (lane P1) fractions, as well as soluble (lane S2) and insoluble (lane P2) fractions obtained by extraction of P1 with NaCl at the concentrations indicated at the top. The lower band in the trypanin blot is likely a degradation product.