Fig 3.

CMF22 is localized to the axoneme. Indirect immunofluorescence was performed on detergent-extracted cytoskeletons from CMF22-HA cells. Samples were stained with anti-HA antibodies to detect CMF22-HA and costained with antibodies against markers for the PFR (PFR2) (A to D, I, and J) or axoneme (trypanin) (E to H, K, and L), as indicated. DNA was visualized with DAPI (blue). Phase-contrast, individual fluorescence, and merged fluorescence images are shown. Regions boxed in red and blue in panel D are enlarged in panels I and J, respectively. Regions boxed in red and blue in panel H are enlarged in panels K and L, respectively. The white arrowheads in panels A to D mark the end of the cell body. Red and green arrowheads in panel J mark the proximal end of PFR and CMF22 staining, respectively. Red and green arrowheads in panel L mark the proximal end of trypanin and CMF22 staining, respectively.