Fig 5.

CMF22 knockdown disrupts motility. (A) Northern blot of RNA prepared from CMF22-UKD cells grown in the absence (−) or presence (+) of Tet, as indicated. (Top) Probe with a DNA fragment unique to the CMF22 ORF; (bottom) rRNA as a loading control. (B) Growth curve of CMF22-UKD grown in the absence or presence of Tet for 2 weeks, as indicated. Data are averages of three independent experiments. (C and D) Motility traces of CMF22-UKD grown in the absence or presence of Tet for 72 h, as indicated. Each line traces the movement of an individual trypanosome over a 30-s time interval.