Fig 9.

CMF22-HA is less stably associated with the axoneme in NDRC mutants. (A) Western blot of the indicated subcellular fractions from trypanin-UTR-knockdown, CMF70-knockdown, or CMF22-ORF-knockdown cells probed with anti-HA, antitrypanin, or antitubulin antibody, as indicated. Fractions correspond to whole-cell lysate (lanes L), nonionic detergent-soluble (lanes S1) and insoluble (lanes P1) fractions, as well as soluble (lanes S2) and insoluble (lanes P2) fractions obtained by extraction of P1 with 1 M NaCl. The lower-molecular-mass bands in the anti-HA and antitrypanin blots are likely degradation products.