Fig 4.

Phenotypes of the syb1-S1 mutant. (A) Wild-type (TY161) and syb1-S1 (TY58) cells were sporulated on SSA medium at 28°C for 2 days. Bar, 10 μm. (B) The syb1-S1 mutant allele carries a single nucleotide change (from C to T), which results in the replacement of cysteine 98 with arginine. TMD, transmembrane domain. (C) Wild-type (TY161) and syb1-S1 (TY58) cells were streaked on complete medium (YEA) and incubated at 25°C or 37°C for 3 days. (D) Sizes of wild-type (TY161) and syb1-S1 (TY58) spores.