Fig 2.

Phylogenetic tree and alignment of amino acid sequences of representative members (34 species) in the Wcs1 protein family. A phylogenetic tree (A) is shown for three phyla of fungi: Ascomycota (blue), Basidiomycota (pink), and Zygomycota (green). Bootstrap values are indicated as percentages. The scale bar indicates an evolutionary distance of 0.06 amino acid substitutions per position. Phylogenetic trees of all members of WC families are shown as Fig. S3 and S4 in the supplemental material. The color scheme in partial alignment of amino acid sequences is used as the default scheme of ClustalX (B). The orange indicates G as glycine. The yellow indicates P as proline. The green indicates polar amino acid residues STQN when those residues were conserved more than 50% or hydrophobic residues ACFHILMVWYP when they were conserved more than 60%. The blue indicates hydrophobic residues ACFHILMVWYP when those residues were conserved more than 60%. The cyan indicates H as histidine and Y as tyrosine when each residue was conserved more than 85% or hydrophobic residues ACFILMVWP when they were conserved more than 60%. The magenta indicates negatively charged residues DE when those residues were conserved more than 50%. The blood orange color indicates positively charged residues KR when those residues were conserved more than 60%. Black bars indicate the amino acid sequences that are well conserved in Ascomycota.