Fig 3.

The effect of wcs genes on stripe formation. (A) Wild-type (wt) yeast cells (NIG2017) and deletion mutants for wcs genes (NIG3001, NIG3002, and NIG3003) were spotted on EMM2 agar plates and incubated at 30°C under 12-h L/12-h D light cycles. (B) EMM2 agar plates with deletion mutants of wcs genes (NIG3001, NIG3002, and NIG3003) were incubated at 30°C until forming a hyphal zone during DD and then were transferred into growth conditions at LL (DD→LL, top). Conversely, another was transferred from growth conditions under LL to DD (LL→DD, bottom). (C) The complementation tests of the deletion mutants of the wcs genes were performed by using the plasmid harboring wcs1 (pSJK-wcs1) or wcs2 (pSJK-wcs2). The deletion mutants (NIG3001 and NIG3002) harboring these plasmids or the vector plasmid pSJK11 were incubated at 30°C under 12-h L/12-h D light cycles. (D) An EMM2 agar plate with yeast strain NIG2017 was incubated at 30°C in the dark until forming a hyphal zone and then illuminated by a blue LED lamp for 1 h. It was further incubated under constant red light (620 nm). After stimulation by blue light, images were taken at 1-h intervals. Images shown are at 1 h and 43 h. Upper and lower halves of the images were combined.