Fig 6.

Effect of temperature shift on hyphal cells. (A) Yeast cells (NIG2017) were spotted on the center of EMM2 agar plates and incubated under DD for more than a week: temperature cycles were 12 h at 30°C and 12 h at 35°C or 12 h at 25°C and 12 h at 30°C. (B) EMM2 agar plates were incubated at 30°C until forming hyphal zones under DD and then were transferred into growth condition at 35°C. Conversely, others were transferred from growth conditions under 35°C to 30°C. (C) Yeast cells (NIG1056) were spotted on the center of YE agar plates and incubated under DD for more than a week: temperature cycles were 12 h at 30°C and 12 h at 32°C. (D) An EMM2 agar plate was incubated under constant blue light with temperature cycles of 12 h at 30°C and 12 h at 35°C. The power of the lamps was 50 lx at a 10-cm distance. (E) Yeast cells of deletion mutants of wcs genes were spotted on EMM2 agar plates and incubated at 30°C with temperature cycles of 12 h at 30°C and 12 h at 35°C. (F) The double deletion mutant of wcs1 and wcs2 genes was spotted on morphology agar plates and incubated at 30°C under a 12-h L/12-h D light cycle (top) with temperature cycles of 12 h at 30°C and 12 h at 35°C (bottom).