Fig 3.

Purification and in vitro activity of CAO-1 on resveratrol and piceatannol. (A) Coomassie-stained SDS acrylamide gel showing CAO-1 purification. Lanes: 1, total lysate of control cells expressing GST (control); 2, total lysate of cells expressing GST-CAO-1; 3, sample 2 after removal of inclusion bodies; 4, nonbinding supernatant of sample 3; 5, eluted CAO-1 protein. The size of the only visible protein in lane 5 is indicated. (B) HPLC analyses of the in vitro assay products obtained after incubation of CAO-1 with resveratrol; UV spectra of the indicated peaks are shown in insets. (C) Resveratrol cleavage reaction and the products identified. (D) HPLC analyses of the in vitro assay products obtained after incubation of CAO-1 with piceatannol; UV spectra of the indicated peaks are shown in insets. mAU, milli-absorbance unit.