Table 1.
Escherichia coli strains, bacteriophages, and plasmids
| Escherichia coli strain, bacteriophage, or plasmid | Genotype or description | Reference, construction |
|---|---|---|
| E. coli strains | ||
| MG1655 | F− λ− ilvG rfb-50 rph | 37 |
| ppGpp0 | As MG1655 but relA256 spoT212 | 38 |
| CF1652 | As MG1655 but relA251::kan | 22 |
| MC1061(ϕ24B) | MC1061 genetic background, bearing the ϕ24B prophage | 39 |
| SCUC34 | 933W Δstx2::catGFP lysogen | 40 |
| SCUC143 | P22 Δstx2::catGFP lysogen | 40 |
| SCUC140 | P27Δstx2::catGFP lysogen | 40 |
| SCUC161 | P32 Δstx2::catGFP lysogen | 40 |
| MG1655(ϕ24B) | MG1655 lysogenic with ϕ24B Δstx2::cat | This work, by lysogenization |
| MG1655(933W) | MG1655 lysogenic with 933W Δstx2::catGFP | This work, by lysogenization |
| MG1655 (P22) | MG1655 lysogenic with P22 Δstx2::catGFP | This work, by lysogenization |
| MG1655(P27) | MG1655 lysogenic with P27 Δstx2::catGFP | This work, by lysogenization |
| MG1655(P32) | MG1655 lysogenic with P32 Δstx2::catGFP | This work, by lysogenization |
| ppGpp0(ϕ24B) | ppGpp0 lysogenic with ϕ24B Δstx2::cat | This work, by lysogenization |
| ppGpp0(933W) | ppGpp0 lysogenic with 933W Δstx2::catGFP | This work, by lysogenization |
| ppGpp0(P22) | ppGpp0 lysogenic with P22 Δstx2::catGFP | This work, by lysogenization |
| ppGpp0(P27) | ppGpp0 lysogenic with P27 Δstx2::catGFP | This work, by lysogenization |
| ppGpp0(P32) | ppGpp0 lysogenic with P32 Δstx2::catGFP | This work, by lysogenization |
| CF1652(ϕ24B) | CF1652 lysogenic with ϕ24B Δstx2::cat | This work, by lysogenization |
| CF1652(933W) | CF1652 lysogenic with 933W Δstx2::catGFP | This work, by lysogenization |
| CF1652(P22) | CF1652 lysogenic with P22 Δstx2::catGFP | This work, by lysogenization |
| CF1652(P27) | CF1652 lysogenic with P27 Δstx2::catGFP | This work, by lysogenization |
| CF1652(P32) | CF1652 lysogenic with P32 Δstx2::catGFP | This work, by lysogenization |
| Phages | ||
| ϕ24B | ϕ24B Δstx2::cat | 39 |
| 933W | 933W Δstx2::catGFP | 40 |
| P22 | P22 Δstx2::catGFP | 40 |
| P27 | P27Δstx2::catGFP | 40 |
| P32 | P32 Δstx2::catGFP | 40 |
| Plasmids | ||
| p933Wcmr | Plasmid derived from phage 933W | 23 |
| p22cmr | Plasmid derived from phage P22 | 23 |
| p27cmr | Plasmid derived from phage P27 | 23 |
| p32cmr | Plasmid derived from phage P32 | 23 |
| pCB104 | Plasmid derived from phage λ | 41 |