Fig 3.
CRP regulation of luxR and ainS promoter activity. Specific fluorescence was generated from the reporters Pcon-gfp (pMTV1), PluxR-gfp (pJLB36), and PainS-gfp (pMTV2) carried within ES114 (gray bars) or crp mutant JB24 (black bars) grown in SWTO with 20 mM glucose. Fluorescence from each strain harboring the promoterless vector pVSV33 was subtracted as the background. Data represent the average specific fluorescence when the culture OD595 was ∼2.0. Averages and standard errors were calculated from replicate flasks (n = 2). An asterisk indicates a significant difference between fluorescence in reporters expressed from ES114 or JB24 cultures, as determined by a Student's t test (P ≤ 0.05).