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RT-PCR transcript analysis of luxR and ainS. Copy number of luxR (A), ainS (B), and lpxA (C) transcripts in 10 ng total cDNA generated from ES114 (wild type) or crp mutant JB24 RNA. Bars indicate standard errors (n = 3). An asterisk indicates a significant difference in transcript number between wild-type and crp mutant transcript copy numbers as determined by a Student's t test (P ≤ 0.0005).
